[HTML][HTML] Neurite outgrowth in PC12 cells: distinguishing the roles of ubiquitylation and ubiquitin-dependent proteolysis
M Obin, E Mesco, X Gong, AL Haas, J Joseph… - Journal of Biological …, 1999 - ASBMB
Nerve growth factor (NGF)-induced neurite outgrowth from rat PC12 cells was coincident
with elevated (≥ 2-fold) levels of endogenous ubiquitin (Ub) protein conjugates, elevated
rates of formation of 125 I-labeled Ub∼ E1 (Ub-activating enzyme) thiol esters and 125 I-
labeled Ub∼ E2 (Ub carrier protein) thiol esters in vitro, and enhanced capacity to
synthesize 125 I-labeled Ub-protein conjugates de novo. Activities of at least four E2s were
increased in NGF-treated cells, including E2 (14K), a component of the N-end rule pathway …
with elevated (≥ 2-fold) levels of endogenous ubiquitin (Ub) protein conjugates, elevated
rates of formation of 125 I-labeled Ub∼ E1 (Ub-activating enzyme) thiol esters and 125 I-
labeled Ub∼ E2 (Ub carrier protein) thiol esters in vitro, and enhanced capacity to
synthesize 125 I-labeled Ub-protein conjugates de novo. Activities of at least four E2s were
increased in NGF-treated cells, including E2 (14K), a component of the N-end rule pathway …
