Requirements for Peptide Binding to the Human Transporter Associated with Antigen Processing Revealed by Peptide Scans and Complex Peptide Libraries (∗)
S Uebel, TH Meyer, W Kraas, S Kienle, G Jung… - Journal of Biological …, 1995 - ASBMB
Antigenic peptides are translocated into the lumen of the endoplasmic reticulum by the
action of the transporter associated with antigen processing (TAP), where they are
subsequently needed for the correct assembly of major histocompatability complex
molecules. The transport function was reconstituted in insect cells by expression of both TAP
genes. On the basis of this overexpression system, substrate selection was analyzed in
detail by a direct bimolecular peptide binding assay. Competition assays with peptide …
action of the transporter associated with antigen processing (TAP), where they are
subsequently needed for the correct assembly of major histocompatability complex
molecules. The transport function was reconstituted in insect cells by expression of both TAP
genes. On the basis of this overexpression system, substrate selection was analyzed in
detail by a direct bimolecular peptide binding assay. Competition assays with peptide …
