Modulation of the Pasteur effect in retinal cells: implications for understanding compensatory metabolic mechanisms
BS Winkler, MW Sauer, CA Starnes - Experimental eye research, 2003 - Elsevier
The purpose of the present experiments was to enhance understanding of the factors that
are critical for the survival of retinal cells exposed to mitochondrial inhibition. Confluent
cultures of Müller cells (rMC-1) and human retinal pigment epithelial cells (hRPE) were
incubated in Dulbecco's minimal essential medium in the presence and absence of 1× 10−
5m Antimycin A, an inhibitor of mitochondrial electron transport. To modulate the rates of
aerobic and anaerobic glycolysis, cells were incubated in media containing varying …
are critical for the survival of retinal cells exposed to mitochondrial inhibition. Confluent
cultures of Müller cells (rMC-1) and human retinal pigment epithelial cells (hRPE) were
incubated in Dulbecco's minimal essential medium in the presence and absence of 1× 10−
5m Antimycin A, an inhibitor of mitochondrial electron transport. To modulate the rates of
aerobic and anaerobic glycolysis, cells were incubated in media containing varying …