Genomic response of hypoxic Müller cells involves the very low density lipoprotein receptor as part of an angiogenic network
Müller cells have recently been found to produce select angiogenic substances. In choosing
a more comprehensive approach, we wanted to study the genomic response of Müller cells
to hypoxia to identify novel angiogenic genes. An established Müller cell line (rMC-1) was
exposed to standard or hypoxic conditions. We analyzed gene expression with three
independent microarrays and determined differential expression levels compared to
normoxia. Selected genes were confirmed by real-time PCR (RTPCR). Subcellular …
a more comprehensive approach, we wanted to study the genomic response of Müller cells
to hypoxia to identify novel angiogenic genes. An established Müller cell line (rMC-1) was
exposed to standard or hypoxic conditions. We analyzed gene expression with three
independent microarrays and determined differential expression levels compared to
normoxia. Selected genes were confirmed by real-time PCR (RTPCR). Subcellular …