Counting colonies of clonogenic assays by using densitometric software
M Niyazi, I Niyazi, C Belka - Radiation oncology, 2007 - Springer
M Niyazi, I Niyazi, C Belka
Radiation oncology, 2007•SpringerClonogenic assays are a useful tool to test whether a given cancer therapy can reduce the
clonogenic survival of tumour cells. A colony is defined as a cluster of at least 50 cells which
can often only be determined microscopically. The process of counting colonies is very
extensive work and so we developed software that is able to count the colonies
automatically from scanned flasks. This software is made freely available by us with a
detailed description how to use and install the necessary features.
clonogenic survival of tumour cells. A colony is defined as a cluster of at least 50 cells which
can often only be determined microscopically. The process of counting colonies is very
extensive work and so we developed software that is able to count the colonies
automatically from scanned flasks. This software is made freely available by us with a
detailed description how to use and install the necessary features.
Abstract
Clonogenic assays are a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumour cells. A colony is defined as a cluster of at least 50 cells which can often only be determined microscopically. The process of counting colonies is very extensive work and so we developed software that is able to count the colonies automatically from scanned flasks. This software is made freely available by us with a detailed description how to use and install the necessary features.
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