[PDF][PDF] Genome-wide, single-cell DNA methylomics reveals increased non-CpG methylation during human oocyte maturation
Stem cell reports, 2017•cell.com
The establishment of DNA methylation patterns in oocytes is a highly dynamic process
marking gene-regulatory events during fertilization, embryonic development, and adulthood.
However, after epigenetic reprogramming in primordial germ cells, how and when DNA
methylation is re-established in developing human oocytes remains to be characterized.
Here, using single-cell whole-genome bisulfite sequencing, we describe DNA methylation
patterns in three different maturation stages of human oocytes. We found that while broad …
marking gene-regulatory events during fertilization, embryonic development, and adulthood.
However, after epigenetic reprogramming in primordial germ cells, how and when DNA
methylation is re-established in developing human oocytes remains to be characterized.
Here, using single-cell whole-genome bisulfite sequencing, we describe DNA methylation
patterns in three different maturation stages of human oocytes. We found that while broad …
Summary
The establishment of DNA methylation patterns in oocytes is a highly dynamic process marking gene-regulatory events during fertilization, embryonic development, and adulthood. However, after epigenetic reprogramming in primordial germ cells, how and when DNA methylation is re-established in developing human oocytes remains to be characterized. Here, using single-cell whole-genome bisulfite sequencing, we describe DNA methylation patterns in three different maturation stages of human oocytes. We found that while broad-scale patterns of CpG methylation have been largely established by the immature germinal vesicle stage, localized changes continue into later development. Non-CpG methylation, on the other hand, undergoes a large-scale, generalized remodeling through the final stage of maturation, with the net overall result being the accumulation of methylation as oocytes mature. The role of the genome-wide, non-CpG methylation remodeling in the final stage of oocyte maturation deserves further investigation.
cell.com